Recombinant Phospho-HER2/ErbB2 (Tyr1221, 1222) Monoclonal Antibody (AN300005L)
For research use only.
| Verified Samples | Verified Samples in WB: SkBr3 |
| Dilution | WB 1:10000-1:200000, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB |
| Clonality | Rabbit Monoclonal |
| Immunogen | A synthetic phosphopeptide corresponding to residues around (Tyr1221, 1222) of human HER2/ErbB2. |
| Abbre | ERBB2 |
| Synonyms | TKR, ERBB, erb-b2 receptor tyrosine kinase, Receptor tyrosine-protein kinase erbB, Tyrosine kinase-type cell surface receptor HER, HER2/ErbB, HER, MLN, ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, erb-b2 receptor tyrosine kinase 2, HER2/ErbB2, MLN19, Metastatic lymph node gene 19 protein, p185, Proto-oncogene Neu, Receptor tyrosine-protein kinase erbB-2, Tyrosine kinase-type cell surface receptor HER2, Neu, erb-B2, CD340, erb-b2 receptor tyrosine kinase 2, HER2, HER-2, HER-2/neu, MLN 19, MLN19, NGL, TKR1, C erb B2/neu protein, CD340 antigen, Cerb B2/neu protein, CerbB2, ENV, ENVW, Erb b2 receptor tyrosine kinase 2, ERVWE1, HER 2, HER 2/NEU, Herstatin, HERV7Q, HERV-7q, HERVW, HERVWENV, HERV-W-ENV, Human epidermal growth factor receptor 2, NEU proto oncogene, neuro/glioblastoma derived oncogene homolog, neuro/glioblastoma derived oncogene homolog (avian), Neuroblastoma/glioblastoma derived oncogene homolog, p185erbB2, Proto-oncogene c-ErbB-2, Tyrosine kinase type cell surface receptor HER2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2, V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog), V erb b2 avian erythroblastic leukemia viral oncoprotein 2, V erb b2 erythroblastic leukemia viral oncogene homolog 2, Verb b2 erythroblastic leukemia viral oncogene homolog 2 |
| Swissprot | |
| Calculated MW | 138 kDa |
| Observed MW |
220 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | 10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5 |
| Purification Method | Protein A |
| Research Areas | Tags & Cell Markers, Signal Transduction, Cancer |
| Clone No. | 1A9 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
