Recombinant Phospho-Tau (S262) Monoclonal Antibody (AN301700L)

For research use only.
Verified Samples | Verified Samples in WB: HeLa |
Dilution | WB 1:500-1:2000 |
Isotype | IgG, κ |
Host | Rabbit |
Reactivity | Human, |
Applications | WB |
Clonality | Monoclonal;Recombinant |
Immunogen | Recombinant human Tau (phospho S262) fragment |
Abbre | Phospho-Tau (S262) |
Synonyms | FTDP, PPP1R, DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, MAPT, Microtubule-Associated Protein Tau, Neurofibrillary Tangle Protein, Paired Helical Filament-Tau, PHF-Tau, pMAPT/pTAU, TAU and MAPT, N/A, AI413597, AW045860, FLJ31424, FTDP 17, G protein beta1/gamma2 subunit interacting factor 1, included, MGC134287, MGC138549, MGC156663, Microtubule associated protein tau, Microtubule associated protein tau isoform 4, Mtapt, Paired helical filament tau, PHF tau, Protein phosphatase 1, pTau, regulatory subunit 103, RNPTAU, Tauopathy and respiratory failure |
Swissprot | |
Calculated MW | 79 kDa |
Observed MW |
50 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Cellular Localization | Cytoplasm, Cytosol, Plasma membrane, Cytoskeleton |
Concentration | 1 mg/mL |
Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
Purification Method | Protein A purified |
Research Areas | Neuroscience, Signal Transduction |
Clone No. | A408 |
Conjugation | Unconjugated |
Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
Shipping | Ice bag |
background | Tau is a neuronal microtubule-associated protein, which is mainly located in the cytoplasm, cytoskeleton and cell membrane. Tau is expressed in neuronal tissues and exists in the form of a variety of alternate splicing subtypes. Its C-terminus is combined with axon microtubules, and the N-terminus is combined with neuroplasmic membrane components to maintain the stability and plasticity of the neuronal cytoskeleton. In neurons, hyperphosphorylated and abnormally glycosylated Tau protein accumulates, causing neurofibrosis and neurodegeneration, such as Alzheimer’s disease (AD), frontotemporal dementia, and chronic traumatic encephalopathy (CTE), Parkinson's disease after encephalitis, etc. |
Other Clones
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Unconjugated
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