Recombinant Phospho-TrkA (Y791) Monoclonal Antibody (AN301752L)
For research use only.
| Verified Samples | Verified Samples in WB: SHSY-5Y, Mouse brain, Rat brain, Mouse brain tissue lysate, then the membrane treated with alkaline phosphatase (50 U/ml) at 37-°C for 1 hour, Mouse brain tissue lysate |
| Dilution | WB 1:1000-1:5000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human TrkA (phospho Y791) fragment |
| Abbre | Phospho-TrkA (Y791) |
| Synonyms | NTRK, NTRK1, MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA, gp140trk, High affinity nerve growth factor receptor, High affinity nerve growth factor receptor precursor, Neurotrophic tyrosine kinase receptor type 1, Oncogene TRK, p140 TrkA, p14-TrkA, Slow nerve growth, Trk A, TRK1-transforming tyrosine kinase protein, Tropomyosin-related kinase A, Tyrosine kinase receptor, Tyrosine kinase receptor A |
| Swissprot | |
| Calculated MW | 87, 92 kDa |
| Observed MW |
120, 145 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Plasma membrane, Endosome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Cancer, Metabolism |
| Clone No. | A460 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | NTRK1 (Neurotrophic Receptor Tyrosine Kinase 1) is a Protein Coding gene. Diseases associated with NTRK1 include Insensitivity to Pain, Congenital, With Anhidrosis and Thyroid Carcinoma, Familial Medullary. Among its related pathways are PI3K/AKT activation and p70S6K Signaling. |
Other Clones
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Other Formats
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Unconjugated
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