Recombinant PI3 Kinase p110 alpha Monoclonal Antibody (AN301630L)

Name: Recombinant PI3 Kinase p110 alpha Monoclonal Antibody
Brand: Elabscience
SKU: AN301630L
Price: 249.0 USD
Availability: InStock

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Recombinant PI3 Kinase p110 alpha Monoclonal Antibody - 1

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	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: HeLa, A431 Verified Samples in IHC: Human breast cancer, Human cervical cancer, Human stomach, Mouse stomach, Rat stomach Verified Samples in IF: HeLa
Dilution	WB 1:500-1:2000, IHC 1:200-1:1000, IF 1:50
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC, IF
Clonality	Monoclonal;Recombinant
Immunogen	Recombinant human PI3 Kinase p110 alpha fragment
Abbre	PI3 Kinase p110 alpha
Synonyms	CWS, PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, PI3K-alpha, 5 bisphosphate 3 kinase 110 kDa catalytic subunit alpha, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha, 5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha (PtdIns-3-kinase subunit p110-alpha, 5-bisphosphate 3-kinase catalytic subunit alpha isoform, caPI3K, MGC142161, MGC142163, p110 alpha, p110alpha, Phosphatidylinositol 3 kinase catalytic 110 KD alpha, Phosphatidylinositol 3 kinase catalytic alpha polypeptide, Phosphatidylinositol 4, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit alpha isoform, Phosphatidylinositol-4, Phosphoinositide 3 kinase catalytic alpha polypeptide, Phosphoinositide 3-kinase alpha, Phosphoinositide-3-kinase catalytic alpha polypeptide, PI3 kinase p110 subunit alpha, PI3Kalpha, PI3KC A, PI3-kinase subunit alpha, PIK3C A, PK3CA, PtdIns 3 kinase p110, PtdIns-3-kinase subunit alpha, PtdIns-3-kinase subunit p110-alpha, Serine/threonine protein kinase PIK3CA
Swissprot	P42336
Calculated MW	110 kDa
Observed MW	120 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Cytosol, Membrane
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Signal Transduction, Cancer, Immunology
Clone No.	A333
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	Phosphoinositide-3-kinase (PI3K) that phosphorylates PtdIns (Phosphatidylinositol), PtdIns4P (Phosphatidylinositol 4-phosphate) and PtdIns(4,5)P2 (Phosphatidylinositol 4,5-bisphosphate) to generate phosphatidylinositol 3,4,5-trisphosphate (PIP3). PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors.