Recombinant PI3-Kinase p110 β Monoclonal Antibody (AN300619L)
For research use only.
| Verified Samples | Verified Samples in WB: RT-4 |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human PI3-Kinase p110 β protein |
| Abbre | PI3-Kinase p110 β |
| Synonyms | PIK3C, PIK3CB, P110BETA, PI3K, PI3KBETA, PIK3C1, 5-bisphosphate 3-kinase 110 kDa catalytic subunit beta, 5-bisphosphate 3-kinase catalytic subunit beta, 5-bisphosphate 3-kinase catalytic subunit beta isoform, DKFZp779K1237, MGC133043, OTTHUMP00000216901, OTTHUMP00000216904, p110 BETA, Phosphatidylinositol 3 kinase catalytic beta polypeptide, Phosphatidylinositol 4, Phosphatidylinositol 4 5 bisphosphate 3 kinase 110 kDa catalytic subunit beta, Phosphatidylinositol 4 5 bisphosphate 3 kinase catalytic subunit beta isoform, phosphatidylinositol-4, Phosphoinositide 3 kinase catalytic beta polypeptide, PI3 kinase p110 subunit beta, PI3K beta, PI3K-beta, PI3KCB, PI3-kinase subunit beta, PK3CB, PtdIns 3 kinase p110, PtdIns-3-kinase subunit beta, PtdIns-3-kinase subunit p110-beta, PK3CB |
| Swissprot | |
| Calculated MW | 123 kDa |
| Observed MW |
110 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus, Interaction with PIK3R2 is required for nuclear localization and export. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Cell Biology, Signal Transduction, Immunology |
| Clone No. | 5A6 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants. |
Other Clones
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Unconjugated
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