Recombinant PI3 Kinase p85 alpha Monoclonal Antibody (AN301629L)
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, C6, CHO-K1, Rat brain, Mouse muscle, MCF-7 Verified Samples in IF: MCF-7 |
| Dilution | WB 1:1000, ICC/IF 1:100-1:200, IP 1:50, FCM 1:100 |
| Isotype | Rabbit IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ICC/IF, FC, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | A synthetic peptide of human PI 3 Kinase p85 alpha |
| Abbre | PI3 Kinase p85 alpha |
| Synonyms | GRB, IMD, AGM, PIK3R, AGM7, GRB1, IMD36, p85, p85-ALPHA, PI3 Kinase p85 alpha, PIK3R1, p85 alpha, P85A, Phosphatidylinositol 3 kinase, Phosphatidylinositol 3 kinase associated p 85 alpha, Phosphatidylinositol 3 kinase regulatory 1, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha, Phosphatidylinositol 3-kinase regulatory subunit alpha, Phosphoinositide 3 kinase, PI3 kinase p85 subunit alpha, PI3K, PI3K regulatory subunit alpha, PI3-kinase regulatory subunit alpha, PI3-kinase subunit p85-alpha, polypeptide 1 (p85 alpha), PtdIns 3 kinase p85 alpha, PtdIns-3-kinase regulatory subunit alpha, PtdIns-3-kinase regulatory subunit p85-alpha, regulatory subunit, regulatory subunit 1 (alpha) |
| Swissprot | |
| Calculated MW | 84 kDa |
| Observed MW |
85 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytosol, Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer, Immunology, Metabolism |
| Clone | C171252 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. |
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