Recombinant PKC zeta Monoclonal Antibody (AN301942L)
For research use only.
| Verified Samples | Verified Samples in WB: 293T, HT-29, Mouse lung |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human PKC zeta fragment |
| Abbre | PKC zeta |
| Synonyms | PKC, PRKCZ, PKC-ZETA, PKC2, 14-3-3-zetaisoform, AI098070, aPKCzeta, C80388, EC 2.7.11.13, included, KPCZ, nPKC zeta, nPKC-zeta, OTTHUMP00000001368, OTTHUMP00000044160, PKC 2, PKC ZETA, Pkcz, PKC-Z, PKCZETA, PKM-zeta, Protein kinase C zeta, Protein kinase C zeta form, Protein kinase C zeta type, Protein Kinase M Zeta, r14-3-3, R74924, zetaPKC |
| Swissprot | |
| Calculated MW | 68 kDa |
| Observed MW |
70 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Endosome |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cancer, Cardiovascular |
| Clone No. | A658 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. |
Other Clones
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Unconjugated
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