Recombinant PKR Monoclonal Antibody (AN300649L)
For research use only.
| Verified Samples | Verified Samples in WB: K562 |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human PKR protein |
| Abbre | PKR |
| Synonyms | PPP1R, EIF2AK1, PKR, PPP1R83, PRKR, Eif2ak2, Double stranded RNA activated protein kinase, double-stranded RNA-activated protein kinase, E2AK2, eIF-2A protein kinase 2, Eukaryotic translation initiation factor 2 alpha kinase 2, Eukaryotic translation initiation factor 2-alpha kinase 2, HGNC:9437, Interferon induced double stranded RNA activated protein kinase, Interferon inducible elF2 alpha kinase, Interferon inducible RNA dependent protein kinase, Interferon-induced, Interferon-inducible RNA-dependent protein kinase, MGC126524, P1/eIF2A protein kinase, P1/eIF-2A protein kinase, p68 kinase, Protein kinase interferon inducible double stranded RNA dependent, Protein kinase RNA activated, Protein kinase RNA-activated, Protein phosphatase 1 regulatory subunit 83, Serine/threonine protein kinase TIK, Tyrosine protein kinase EIF2AK2 |
| Swissprot | |
| Calculated MW | 62 kDa |
| Observed MW |
62 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus, Cytoplasm, perinuclear region, Nuclear localization is elevated in acute leukemia, myelodysplastic syndrome (MDS), melanoma, breast, colon, prostate and lung cancer patient samples or cell lines as well as neurocytes from advanced Creutzfeldt-Jakob disease patients. |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Signal Transduction |
| Clone No. | 8D9 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | The protein encoded by this gene is a serine/threonine protein kinase that is activated by autophosphorylation after binding to dsRNA. The activated form of the encoded protein can phosphorylate translation initiation factor EIF2S1, which in turn inhibits protein synthesis. This protein is also activated by manganese ions and heparin. Three transcript variants encoding two different isoforms have been found for this gene. |
Other Clones
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Unconjugated
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