Recombinant PRK2 Monoclonal Antibody (AN301803L)
For research use only.
| Verified Samples | Verified Samples in WB: 293T, HeLa, HepG2, NIH/3T3, PC-12 |
| Dilution | WB 1:500-1:2000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human PRK2 fragment |
| Abbre | PRK2 |
| Synonyms | STK, PRO, PKN, PRK, PRKCL, Pak, PAK2, PRK2, PRKCL2, PRO2042, Pak-2, STK7, PKN2 |
| Swissprot | |
| Calculated MW | 112 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Cytoplasm, cytoskeleton |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Cell Biology, Cancer |
| Clone No. | A515 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | PRK2 is a member of the protein kinase C-related kinases (PRKs), a subfamily of Ser/Thr-specific kinases with a catalytic domain highly homologous to the PKC family. PKC-related serine/threonine-protein kinase and Rho/Rac effector protein participate in specific signal transduction responses in the cell. PRK2 plays a role in regulating cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion, and transcription activation signaling processes. Among its related pathways are signaling by Rho GTPases and signaling by GPCR. Gene Ontology (GO) annotations related to this gene include RNA binding and protein kinase activity. |
Other Clones
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