Recombinant PTGER2 Monoclonal Antibody (AN301641L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, JAR, F9, Rat liver Verified Samples in IHC: Human placenta, Mouse liver, Rat lung Verified Samples in IF: MCF7 |
| Dilution | WB 1:500-1:1000, IHC 1:50-1:100, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human PTGER2 fragment |
| Abbre | PTGER2 |
| Synonyms | Ptger, PTGER2, EP2, PE2R2, PGE receptor EP2 subtype, PGE2 receptor EP2 subtype, Prostaglandin E receptor 2 EP2 subtype, Prostaglandin E receptor 2 subtype EP2, Prostaglandin E receptor 2 subtype EP2 53kDa, Prostaglandin E2 receptor, Prostaglandin E2 receptor 2, Prostaglandin E2 receptor EP2 subtype, Prostanoid EP2 receptor |
| Swissprot | |
| Calculated MW | 52 kDa |
| Observed MW |
56 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Signal Transduction |
| Clone No. | A344 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. The subsequent raise in intracellular cAMP is responsible for the relaxing effect of this receptor on smooth muscle. |
Other Clones
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Other Formats
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Unconjugated
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