Recombinant QSOX1 Monoclonal Antibody (AN301645L)
For research use only.
| Verified Samples |
Verified Samples in WB: MCF-7, MDA-MB-231, T47D, 293T Verified Samples in FCM: MDA-MB-231 Verified Samples in IP: 293T cells extracts |
| Dilution | WB 1:500-1:1000, FCM 1:100, IP 1:25-1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, FCM, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human QSOX1 fragment |
| Abbre | QSOX1 |
| Synonyms | UNQ, PRO, QSOX, QSCN, QSOX1, Q6, QSCN6, UNQ2520, PRO6013, quiescin sulfhydryl oxidase 1 |
| Swissprot | |
| Calculated MW | 83 kDa |
| Observed MW |
67 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Extracellular area, Golgi apparatus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology |
| Clone No. | A348 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | QSOX1 belongs to the family of FAD-dependent sulfhydryl oxidases and is an atypical disulfide catalyst. QSOX1 is mainly located in the Golgi apparatus and endoplasmic reticulum (ER) in human embryonic fibroblasts, and it cooperates with protein disulfide isomerase (PDI) to help fold new-born proteins in the cell. QSOX1 is mainly expressed in the heart, placenta, lung, liver, skeletal muscle, and pancreas, and is very weakly expressed in the brain and kidneys. Studies have found that the increase of QSOX1 expression in tumor cells enables tumor cells to actively circumvent the mechanism of apoptosis mediated by reactive oxygen species. |
Other Clones
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Unconjugated
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