Recombinant Rad51 Monoclonal Antibody (AN301776L)
-
-
-
- +2
For research use only.
| Verified Samples |
Verified Samples in WB: Jurkat, NIH/3T3, C6 Verified Samples in IHC: Human testis, Rat testis Verified Samples in IF: Hela, Jurkat |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:100, IF 1:100-1:200 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Rad51 fragment |
| Abbre | Rad51 |
| Synonyms | RAD, BRCC, HRAD, MRMV, DNA repair protein RAD51 homolog, HsRad, RAD51, BRCC5, FANCR, HRAD51, HsRad51, HsT16930, MRMV2, RAD51A, RECA, DNA repair protein RAD51 homolog 1, BRCA1/BRCA2 containing complex, BRCC 5, DNA repair protein rhp51, E. coli, E. coli) (S. cerevisiae), homolog of, Rad 51, RAD51 homolog, RAD51 homolog (RecA homolog, RAD51 homolog A, RAD51 recombinase, RecA like protein, Recombination protein A, S. cerevisiae, subunit 5 |
| Swissprot | |
| Calculated MW | 37 kDa |
| Observed MW |
37 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling |
| Clone No. | A484 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | DNA repair protein RAD51 homolog 1 (Rad51) plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Rad51 binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Rad51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
