Recombinant Rat AST protein (His Tag) (PDMR100007)

For research use only.
Synonyms | Aspartate aminotransferase, Cysteine aminotransferase, Cysteine transaminase, Glutamate oxaloacetate transaminase 1, Got1, Transaminase A, cAspAT, cCAT, cytoplasmic |
Species | Rat |
Expression Host | HEK293 Cells |
Sequence | Met1-Gln413 |
Accession | P13221 |
Calculated Molecular Weight | 45.3 kDa |
Observed Molecular Weight | 44 kDa |
Tag | C-His |
Bio-activity | Not validated for activity |
Purity | > 90% as determined by reducing SDS-PAGE. |
Endotoxin | < 1.0 EU/mg of the protein as determined by the LAL method |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
Background | Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain. |
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