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Recombinant Rat AST protein (His Tag) (PDMR100007)

All Size Price Qty
500μg $ 1440.00
100μg $ 488.00
20μg $ 158.00
1mg $ 2340.00
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For research use only.

Synonyms Aspartate aminotransferase, Cysteine aminotransferase, Cysteine transaminase, Glutamate oxaloacetate transaminase 1, Got1, Transaminase A, cAspAT, cCAT, cytoplasmic
Species Rat
Expression Host HEK293 Cells
Sequence Met1-Gln413
Accession P13221
Calculated Molecular Weight 45.3 kDa
Observed Molecular Weight 44 kDa
Tag C-His
Bio-activity Not validated for activity
Purity > 90% as determined by reducing SDS-PAGE.
Endotoxin < 1.0 EU/mg of the protein as determined by the LAL method
Storage Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
Shipping This product is provided as lyophilized powder which is shipped with ice packs.
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Reconstitution It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Background Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.
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