Recombinant Rat IGFBP-3 protein (His Tag) (PDMR100028)
For research use only.
| Synonyms | IBP-3, IGF-binding protein 3, IGFBP-3, Igfbp3, Insulin-like growth factor-binding protein 3 |
| Species | Rat |
| Expression Host | HEK293 Cells |
| Sequence | Met1-Gln292 |
| Accession | P15473 |
| Calculated Molecular Weight | 32.0 kDa |
| Observed Molecular Weight | 42-55 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 90% as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU/mg of the protein as determined by the LAL method |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol. |
| Reconstitution | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis. |
| Background | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Also exhibits IGF-independent antiproliferative and apoptotic effects mediated by its receptor TMEM219/IGFBP-3R. Promotes testicular germ cell apoptosis. |
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