Recombinant RBX1 Monoclonal Antibody (AN301789L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, HepG2, HEK-293, NIH/3T3, PC-12 Verified Samples in IHC: Human bladder cancer, Mouse stomach, Rat stomach Verified Samples in IF: HeLa, NIH/3T3 Verified Samples in IP: HeLa cells extracts |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:500, IF 1:50, IP 1:50-100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human RBX1 fragment |
| Abbre | RBX1 |
| Synonyms | RNF, ROC, RBX, RNF75, ROC1, RBX1, BA554C12.1, E3 ubiquitin-protein ligase RBX1, FLJ60363, MGC13357, MGC1481, OTTHUMP00000028983, Protein ZYP, Rbx 1, Regulator of cullins 1, Ring box 1, Ring box 1 E3 ubiquitin protein ligase, RING box protein 1, RING finger protein, RING finger protein 75, RING-box protein 1, Ringbox protein 1, RNF 75, ROC 1, ZYP protein |
| Swissprot | |
| Calculated MW | 12 kDa |
| Observed MW |
12 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Nucleus |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling |
| Clone No. | A501 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | RBX1 is a RING finger-like domain-containing protein. This protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. |
Other Clones
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Other Formats
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Unconjugated
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