Recombinant RPS3 Monoclonal Antibody (AN301957L)
For research use only.
| Verified Samples | Verified Samples in WB: HL-60, MCF-7, PC-3, HepG2, RAW264.7, NIH/3T3 |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human RPS3 fragment |
| Abbre | RPS3 |
| Synonyms | RPS, OK/SW-cl, RPS3, S3, OK/SW-cl.26, 40S ribosomal protein S3, fb13d09, FLJ26283, FLJ27450, IMR 90 ribosomal protein S3, MGC56088, MGC87870, OTTHUMP00000229804, OTTHUMP00000229805, OTTHUMP00000229874, OTTHUMP00000229877, OTTHUMP00000229878, OTTHUMP00000229879, OTTHUMP00000229880, OTTHUMP00000229882, OTTHUMP00000229883, OTTHUMP00000229886, Ribosomal protein S3, RS3, wu:fb13d09, zgc:56088 |
| Swissprot | |
| Calculated MW | 27 kDa |
| Observed MW |
27 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Cell Biology, Epigenetics and Nuclear Signaling |
| Clone No. | A673 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Ribosomal protein S3 (RPS3) is a component of the 40S ribosomal subunit and is involved in translation. HSP90 interacts with both the amino-terminus and carboxy-terminus of RPS3, preventing its ubiquitination and degradation and thereby retaining the integrity of the ribosome. RPS3 has also been shown to function as an endonuclease during DNA damage repair. Furthermore, overexpression of RPS3 sensitizes lymphocytic cells to cytokine-induced apoptosis, indicating a third role for RPS3 during apoptosis. |
Other Clones
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Unconjugated
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