Recombinant SAA4 Monoclonal Antibody (AN300231P)
For research use only.
| Verified Samples |
Verified Samples in WB: 293T Verified Samples in FCM: HepG2 Verified Samples in IF: HepG2 |
| Dilution | WB 1:500-1:2000, FCM 1:25-1:100, ICC/IF 1:20-1:100, |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, FCM, ICC/IF |
| Clonality | Monoclonal |
| Immunogen | Recombinant Human SAA4 protein |
| Abbre | SAA4 |
| Synonyms | Amyloid A, C SAA, Constitutively expressed serum amyloid A protein, C-SAA, SAA 4, SAA4 protein, SAA4_HUMAN, serum, Serum amyloid A 4 protein, Serum amyloid A4 constitutive, Serum amyloid A-4 protein, CSAA, SAA4, serum, Constitutively expressed serum amyloid A protein (C-SAA) |
| Swissprot | |
| Calculated MW | 15 kDa |
| Observed MW |
15 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | 0.2 μm filtered solution in PBS |
| Purification Method | Protein A |
| Research Areas | Cardiovascular, Metabolism |
| Clone No. | 3F8 |
| Conjugation | Unconjugated |
| Storage | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles. |
| Shipping | Ice bag |
| background | SAA4 is a member of the SAA family. SAA proteins are family of apolipoproteins of high density lipoprotein (HDL). They can be separated into two distinct groups. First group (SAA1, SAA2, and SAA3) consists of acute phase reactant whose expression level increase in the blood in a response to trauma, infection, inflammation, and neoplasia. These acute phase SAAs associates with HDL during inflammation and remodel the HDL particle by displacing Apo-A1. The second distinct group consists of SAA4 and SAA5 which exist as the minor apolipoproteins on HDL, but this group of SAA constitutes more than 90% of all the SAA during homeostasis, and it is thought to play a role in the normal functioning of the HDL particle. SAA4 is a constitutively expressed protein which expressed only in humans and mice. It is connected almost completely with lipoproteins of the high density range. The physiological function of SAA4 is unknown, and its serum concentration has no association with those of other major apolipoproteins. |
Other Clones
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Unconjugated
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