Recombinant SARS-CoV Nucleoprotein / NP Protein (His Tag) (PKSV030248)
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For research use only.
| Synonyms | coronavirus NP Protein, coronavirus Nucleocapsid Protein, coronavirus Nucleoprotein Protein, cov np Protein, ncov NP Protein, novel coronavirus NP Protein, novel coronavirus Nucleocapsid Protein, novel coronavirus Nucleoprotein, novel coronavirus Nucleoprotein Protein, NP Protein, Nucleocapsid Protein, Nucleoprotein Protein, SARS |
| Species | SARS |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Met1-Ala422 |
| Accession | NP_828858.1 |
| Calculated Molecular Weight | 47.5 kDa |
| Observed Molecular Weight | 47.1 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Form | Lyophilized powder |
| Purity | > 80 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. |
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