Recombinant SFTPA1+SFTPA2 Monoclonal Antibody (AN301822L)
For research use only.
| Verified Samples |
Verified Samples in WB: Human lung, Rat lung, Mouse lung Verified Samples in IHC: Human lung |
| Dilution | WB 1:1000-1:3000, IHC 1:200-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human SFTPA1 + SFTPA2 fragment |
| Abbre | SFTPA1+SFTPA2 |
| Synonyms | Collectin-4, COLEC4, Collectin-5, COLEC5, PSAP, SFTP1, SFTPA, SFTPA1B, SFTPA2B |
| Swissprot | |
| Calculated MW | 27 kDa |
| Observed MW |
27 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Extracellular matrix, Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Clone No. | A534 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Human SP-A is expressed in alveolar epithelial type II cells and in tracheal and bronchial submucosal gland cells in lung as well as in other tissues. SFTPA1(SP-A1) and SFTPA2(SP-A2) are expressed in the type II cells; SP-A2 is expressed in human tracheal and bronchial submucosal gland cells. The presence of SP-A in non-ciliated bronchiolar epithelial cells has been inconsistent for human lungs, but consistent positive staining of SP-A has been observed in the non-ciliated bronchiolar epithelial cells in rodents. |
Other Clones
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Other Formats
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Unconjugated
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