Recombinant Synuclein-α Monoclonal Antibody (AN300624L)
For research use only.
| Verified Samples | Verified Samples in WB: Hela |
| Dilution | WB 1:2000-1:10000 |
| Isotype | IgG,κ |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant Human Synuclein-α protein |
| Abbre | Synuclein-α |
| Synonyms | Fetal liver kinase, Protein-tyrosine kinase receptor flk, Vascular endothelial growth factor receptor, PD1, NACP, PARK1, PARK4, SNCA, SNC-A, aSYN, CD309, Fetal liver kinase 1, FLK1, FLK-1, KDR, Kinase insert domain receptor, Protein-tyrosine kinase receptor flk-1, Vascular endothelial growth factor receptor 2, VEGFR, VEGFR2, VEGFR-2, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, PARK1, alpha (non A4 component of amyloid precursor), Alpha synuclein, alphaSYN, isoform NACP140, Lewy body) 4, MGC105443, MGC110988, MGC127560, MGC64356, Non A beta component of AD amyloid, Non A4 component of amyloid, Non A4 component of amyloid precursor, Non-A4 component of amyloid, Non-A-beta component of alzheimers disease amyloid, OTTHUMP00000218549, OTTHUMP00000218551, OTTHUMP00000218552, OTTHUMP00000218553, OTTHUMP00000218554, PARK 1, PARK 4, Parkinson disease (autosomal dominant, Parkinson disease familial 1, precursor of, Snca synuclein, SYN, Synuclein, Synuclein alpha, Synuclein alpha 140, SYUA |
| Swissprot | |
| Calculated MW | 14 kDa |
| Observed MW |
18 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm, Membrane, Nucleus, Cell junction, synapse, Secreted, Cell projection, axon, Membrane-bound in dopaminergic neurons (PubMed:15282274). Expressed and colocalized with SEPTIN4 in dopaminergic axon terminals, especially at the varicosities (By similarity). |
| Tissue Specificity | Bone marrow, brain |
| Concentration | 0.2 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A |
| Research Areas | Neuroscience |
| Clone No. | 5A11 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. |
Other Clones
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Unconjugated
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