Recombinant TGF beta 1 Monoclonal Antibody (AN301902L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Saos-2, K562, Mouse spleen, Rat spleen Verified Samples in IHC: Human spleen, Mouse spleen, Rat spleen |
| Dilution | WB 1:1000, IHC 1:500-1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human TGF beta 1 fragment |
| Abbre | TGF beta 1 |
| Synonyms | DPD, Transforming growth factor beta, TGF-B, TGFB1, CED, DPD1, LAP, TGFB, TGFbeta, TGF-β, Cartilage-inducing factor, Differentiation inhibiting factor, IBDIMDE, TGF-B1, TGF-beta 1, TGF-beta-1, TGF-β1, Transforming growth factor beta-1 proprotein, Latency-associated peptide, Transforming growth factor beta-1, beta 1, Prepro transforming growth factor beta 1, TGF beta, TGF beta 1, TGF beta 1 protein, Tgfb-1, TGF-beta 1 protein, TGFbeta1, TGF-beta1, TGF-beta-5, transforming growth factor, Transforming Growth Factor b1, Transforming Growth Factor beta 1, Transforming growth factor beta 1a, Transforming Growth Factor-1 |
| Swissprot | |
| Calculated MW | 44 kDa |
| Observed MW |
44, 10 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Stem Cells, Cancer, Metabolism, Cardiovascular, Cell Biology |
| Clone No. | A618 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Transforming growth factor-β (TGF-β) superfamily members are critical regulators of cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis. TGF-β elicits signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII). Type I and type II receptors are serine/threonine kinases that form a heteromeric complex. In response to ligand binding, the type II receptors form a stable complex with the type I receptors allowing phosphorylation and activation of type I receptor kinases. The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain. Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex. The only known direct TGF-β signaling effectors are the Smad family proteins, which transduce signals from the cell surface directly to the nucleus to regulate target gene transcription. |
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