Recombinant Thymidylate Synthase Monoclonal Antibody (AN301674L)
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For research use only.
| Verified Samples |
Verified Samples in WB: HeLa, Jurkat, Raw264.7, 293T, Raji Verified Samples in IHC: Human colon cancer, Human ovarian cancer Verified Samples in IF: Jurkat Verified Samples in IP: Jurkat cells extracts |
| Dilution | WB 1:500-1:1000, IHC 1:100-1:1000, IF 1:50, IP 1:25-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, |
| Applications | WB, IHC, IF, IP |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human Thymidylate Synthase fragment |
| Abbre | Thymidylate Synthase |
| Synonyms | HST, OK/SW-cl, TYMS, HST422, TMS, TS, thymidylate synthase, OK/SW-cl.29, HST422, thymidylate synthase, TMS, thymidylate synthetase |
| Swissprot | |
| Calculated MW | 36 kDa |
| Observed MW |
34 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Nucleus, Cytoplasm, Mitochondrion, Mitochondrion matrix, Mitochondrion inner membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Epigenetics and Nuclear Signaling, Metabolism |
| Clone No. | A377 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | Thymidylate synthase (TS) is a key enzyme in the synthesis of 2'-deoxythymidine-5'-monophosphate, an essential precursor for DNA biosynthesis. Thymine nucleotide synthase is a key enzyme in the de novo synthesis pathway of mitochondrial thymine. For this reason, it is a key target for cancer chemotherapy, and considered to be the main target of 5-fluorouric acid, 5-fluoro-2'-deoxyribonucleic acid and some folate compounds. |
Other Clones
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Other Formats
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Unconjugated
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