Recombinant TSHB Monoclonal Antibody (AN301422L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Rat pituitary Verified Samples in IHC: Rat pituitary Verified Samples in IF: HeLa, U-2 OS Verified Samples in FCM: HeLa |
| Dilution | WB 1:500-1:2000, IHC 1:100-1:500, IF 1:1000-1:2000, FCM 1:50-1:100 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, |
| Applications | WB, IHC, IF, FCM |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human TSHB fragment |
| Abbre | TSHB |
| Synonyms | CHNG, TSHB, TSH-B, TSH-BETA, beta precursor, CHNG4, Thyroid stimulating hormone, Thyroid stimulating hormone beta subunit, Thyrotropin, Thyrotropin beta subunit |
| Swissprot | |
| Calculated MW | 16 kDa |
| Observed MW |
16 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cytoplasm |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Signal Transduction, Neuroscience, Cancer, Metabolism |
| Clone No. | A117 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | TSHB (Thyroid Stimulating Hormone Subunit Beta) is a Protein Coding gene. Diseases associated with TSHB include Hypothyroidism, Congenital, Nongoitrous, 4 and Central Congenital Hypothyroidism. Among its related pathways are Signaling by GPCR and Peptide hormone metabolism. Gene Ontology (GO) annotations related to this gene include hormone activity. An important paralog of this gene is FSHB. |
Other Clones
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Other Formats
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Unconjugated
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