Recombinant VPAC1 Monoclonal Antibody (AN301687L)
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For research use only.
| Verified Samples |
Verified Samples in WB: Mouse small intestine, Rat small intestine Verified Samples in IHC: Human tonsil Verified Samples in IF: Raji, EL4 |
| Dilution | WB 1:500-1:2000, IHC 1:50-1:100, IF 1:50 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Rat, Mouse |
| Applications | WB, IHC, IF |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Recombinant human VPAC1 fragment |
| Abbre | VPAC1 |
| Synonyms | RDC, HVR, VAPC, VPAC, VIP-R, VIPR1, HVR1, II, PACAP-R-2, PACAP-R2, RDC1, V1RG, VAPC1, VIP-R-1, VIPR, VIRG, VPAC1, VPAC1R, VPCAP1R, PACAP type II receptor, Pituitary adenylate cyclase-activating polypeptide type II receptor |
| Swissprot | |
| Calculated MW | 90 kDa |
| Observed MW |
90 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Neuroscience, Tags & Cell Markers, Signal Transduction, Cancer, Cardiovascular |
| Clone No. | A390 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | VPAC1 is the receptor for vasoactive intestinal peptides. This small neuropeptide is involved in regulating smooth muscle relaxation, the water and ion flux of the lung and intestinal epithelium, promoting neuron growth and survival, and regulating immune function. The activity of VPAC1 is regulated by G protein. VPAC1 interacts with citrulline nucleotide binding protein-related membrane receptors to activate adenate cyclase. |
Other Clones
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Other Formats
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Unconjugated
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