Recombinant WFDC2 Monoclonal Antibody (AN302016L)
For research use only.
| Verified Samples | Verified Samples in WB: HeLa,?TH-29, Mouse liver |
| Dilution | WB 1:1000 |
| Isotype | IgG, κ |
| Host | Rabbit |
| Reactivity | Human, Mouse |
| Applications | WB |
| Clonality | Monoclonal;Recombinant |
| Immunogen | Peptide. This information is proprietary to PTMab. |
| Abbre | WFDC2 |
| Synonyms | WAP, WFDC, EDDM, Epididymal Secretory Protein E, Putative Protease Inhibitor WAP, Major Epididymis-Specific Protein E, WAP Four-Disulfide Core Domain Protein, dJ461P, WFDC2, EDDM4, HE4, WAP5, dJ461P17.6, Epididymal Secretory Protein E4, Major Epididymis-Specific Protein E4, Putative Protease Inhibitor WAP5, WAP Four-Disulfide Core Domain Protein 2, WAP four-disulfide core domain 2 |
| Swissprot | |
| Calculated MW | 13 kDa |
| Observed MW |
22 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Secreted |
| Concentration | 1 mg/mL |
| Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant. |
| Purification Method | Protein A purified |
| Research Areas | Tags and Cell Markers |
| Clone No. | A736 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | Ice bag |
| background | WFDC2 (WAP four-disulfide core domain protein 2), also known as human epididymal secretory protein E4 (HE4), is a small secreted broad range protease inhibitor. WFDC2 is a diagnostic marker in ovarian, endometrial and other cancers, and can be detected in patient serum. WFDC2 expression promotes tumor growth, contributes to metastasis and epithelial mesenchymal transition (EMT), and regulates angiogenesis and immunogenic signaling in the tumor microenvironment. |
| Cat.No. | Product Name | Clone No. |
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