Recombinant WT1 Monoclonal Antibody (AN302022L)

Name: Recombinant WT1 Monoclonal Antibody
Brand: Elabscience
SKU: AN302022L
Price: 249.0 USD
Availability: InStock

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	100μL	$ 380.00	- +
	50μL	$ 249.00	- +

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Verified Samples	Verified Samples in WB: LNCaP (negative?control),?K562, Mouse testis,?Rat testis Verified Samples in IHC: Human kidney, Human testis, Human Wilms tumor, Mouse testis
Dilution	WB 1:2000-1:10000, IHC 1:100
Isotype	IgG, κ
Host	Rabbit
Reactivity	Human, Rat, Mouse
Applications	WB, IHC
Clonality	Monoclonal;Recombinant
Immunogen	Peptide. This information is proprietary to PTMab.
Abbre	WT1
Synonyms	AWT, FWT, NPHS, EWS-WT, WIT, WT1, AWT1, EWS-WT1, GUD, NPHS4, WAGR, WIT-2, WT33, FWT1, Wilms Tumor, Wilms tumor 1, Wilms' tumor gene, Wilms tumor protein, Wilms' tumor protein, WIT 2, WIT2, WT, WT 1, WIT 2, WT 1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1, Wilms Tumor, Wilms tumor protein, Wilms' tumor gene, Wilms' tumor protein, WIT2, WT, WT1, WT1, WT33
Swissprot	P19544
Calculated MW	49 kDa
Observed MW	52 kDa The actual band is not consistent with the expectation. Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.
Cellular Localization	Cytoplasm, Nucleus
Concentration	1 mg/mL
Buffer	PBS, 50% glycerol, 0.05% Proclin 300, 0.05% protein protectant.
Purification Method	Protein A purified
Research Areas	Tags & Cell Markers, Epigenetics and Nuclear Signaling, Cancer, Developmental Biology
Clone No.	A742
Conjugation	Unconjugated
Storage	Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.
Shipping	Ice bag
background	Wilms' Tumor protein (WT1) is a transcription factor named from Wilms' Tumor 1, an embryonal malignancy of the kidneys that is caused by mutations in the WT1 gene. It is highly important in development, particularly of the genitourinary system, and mutations and dysregulation of expression of WT1 result in a variety of syndromes affecting the genitourinary system and other tissues.WT1 has a myriad of biological functions and a host of interacting partners and target genes. It can behave as a transcriptional activator, or a repressor, and can act as an oncogene or a tumor suppressor. It exerts influence over the epigenetic landscape, and also has post translational influence of gene expression through RNA interactions. The diverse biological roles of WT1 have been attributed to the existence of multiple isoforms and post translation modifications of the protein.