Recombinant ZIKV (strain Zika SPH2015) NS5 protein (His Tag) (PKSV030274)
For research use only.
| Synonyms | NS5 protein, Zika virus, strain Zika SPH2015 |
| Species | ZIKV |
| Expression Host | Baculovirus-Insect Cells |
| Sequence | Lys2772-Leu3423 |
| Accession | ALU33341.1 |
| Calculated Molecular Weight | 76.7 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation |
Lyophilized from sterile 20 mM PB,100 mM NaCl,50% glycerol, pH 7.0, 0.5 mM TCEP. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
| Background | Zika virus NS5 is invloved in methytransferase and RNA guanylytransferase activities and capping and synthesis of RNA. And, NS5 is also a RNA-dependent RNA polymerase. |
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