For research use only.
| Verified Samples |
Verified Samples in WB: HT29, HepG2, HT1080, HeLa |
| Dilution | WB 1:500-1:2000 |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human ROBO4 (NP_061928.4). |
| Synonyms | ECSM4, MRB, ROBO4 |
| Swissprot | |
| Calculated MW | 75 kDa/98 kDa/107 kDa |
| Observed MW |
130 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Extracellular region or secreted, extracellular exosome, Other locations: integral component of membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Neuroscience |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | ROBO4 (Roundabout Guidance Receptor 4) is a Protein Coding gene. Diseases associated with ROBO4 include Aortic Valve Disease 3 and Diaphragmatic Hernia, Congenital. Among its related pathways are Development Slit-Robo signaling. An important paralog of this gene is SDK1. |
Other Clones
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