S100A11 Polyclonal Antibody (D-AB-10187L)
For research use only.
| Verified Samples |
Verified Samples in WB: PC-3 Verified Samples in IHC: Human lung cancer |
| Dilution | WB 1:500-1:1000, IHC 1:200-1:400 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Recombinant Human S100A11 protein expressed by E.coli |
| Abbre | S100A11 |
| Synonyms | Calgizzarin, Epididymis secretory protein Li 43, HEL S 43, MLN 70, MLN70, Metastatic lymph node gene 70 protein, N-terminally processed, Protein S100 A11, Protein S100-A11, Protein S100-C, Protein S100A11, Protein S100C, S100, S100 A11, S100 calcium binding protein A11 |
| Swissprot | |
| Calculated MW | 12 kDa |
| Observed MW |
11 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.05% proclin 300, 1% protective protein and 50% glycerol,pH7.4 |
| Purification Method | Antigen Affinity Purification |
| Research Areas | Cancer, Cell Biology, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | S100A11,also named as MLN 70,Calgizzarin and S100C,Belongs to the S-100 family. It facilitates the differentiation and the cornification of keratinocytes. The naming of S100A11 systematically arises from its membership of the S100 protein family,named after their solubility in 100% saturated ammonium sulfate solution. First discovered in 1989,S100A11 has since been proposed to have direct biological functions in an assortment of physiological processes such as endo- and exocytosis,regulation of enzyme activity,cell growth and regulation,apoptosis and inflammation. |
Other Clones
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Unconjugated
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