Selenium Colorimetric Assay Kit (E-BC-K776-M)
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For research use only.
| Detection Principle |
Selenium is an essential microelements in the human body. It participates in many metabolic pathways of the human body, and has the functions of delaying aging, enhancing immunity and preventing many diseases. The intake of the selenium through daily diet. Excessive intake of selenium can cause selenium poisoning, leading to multiple organ damage, and insufficient intake can also cause a variety of diseases, such as Kashin-beck disease, etc. The detection principle of this kit: under acidic conditions, selenium is reduced to tetravalent selenium, and then reacts with the chromogenic agent to produce yellow complex, which increase the absorbance at 420 nm, and the color development intensity is proportional to the content of tetravalent selenium in the sample. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (415-425 nm, optimum wavelength: 420 nm) |
| Research Area | Inorganic Ions |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.06 mmol/L |
| Detection Range | 0.06-16.00 mmol/L |
| Precision | inter-assay CV: 0.5-0.7% | intra-assay CV: 0.3-0.4% |
| Sample Volume | 100-150 μL |
| Assay Time | 50 min |
| Cat.No. | Product Name | Clone No. |
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