Serpin E1/PAI-1 Polyclonal Antibody(Capture/Detector) (AN000040P)

For research use only.
Verified Samples |
Verified Samples in WB: Rat placenta Verified Samples in ELISA: Recombinant Rat Serpin E1/PAI-1 protein, Rat serum, Rat plasma |
Dilution | WB 1:500-1:1000, ELISA Capture 2-8 μg/mL, ELISA Detector 0.1-0.4 μg/mL |
Isotype | Rabbit IgG |
Host | Rabbit |
Reactivity | Rat |
Applications | WB, ELISA Capture/Detector |
Clonality | Polyclonal |
Immunogen | Recombinant Rat Serpin E1/PAI-1 protein expressed by Mammalian |
Abbre | Serpin E1/PAI-1 |
Synonyms | PLasminogen activator inhibitor 1, PAI-1, EndotheLiaL pLasminogen activator inhibitor, Serpin E1, SERPINE1, PLANH1 |
Swissprot | |
Calculated MW | 45 kDa |
Observed MW |
47 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
Concentration | 1 mg/mL |
Buffer | Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300. |
Purification Method | Antigen Affinity Purification |
Conjugation | Unconjugated |
Storage | Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles. |
Shipping | The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended. |
background | Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PlAT) and urokinase-type plasminogen activator (PlAU). As PlAT inhibitor,it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PlAU inhibitor,it is involved in the regulation of cell adhesion and spreading.Acts as a regulator of cell migration,independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells,and regulates odontoblast differentiation and dentin formation during odontogenesis. |
Other Clones
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Unconjugated
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