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Serpin E1/PAI-1 Polyclonal Antibody(Capture/Detector) (AN000040P)

All Size Price Qty
100μg $ 350.00
25μg $ 130.00
1mg Inquire /
Add to cart

For research use only.

Verified Samples Verified Samples in WB: Rat placenta
Verified Samples in ELISA: Recombinant Rat Serpin E1/PAI-1 protein, Rat serum, Rat plasma
Dilution WB 1:500-1:1000,  ELISA Capture 2-8 μg/mL,  ELISA Detector 0.1-0.4 μg/mL
Isotype Rabbit IgG
Host Rabbit
Reactivity Rat
Applications WB,  ELISA Capture/Detector
Clonality Polyclonal
Immunogen Recombinant Rat Serpin E1/PAI-1 protein expressed by Mammalian
Abbre Serpin E1/PAI-1
Synonyms PLasminogen activator inhibitor 1,  PAI-1,  EndotheLiaL pLasminogen activator inhibitor,  Serpin E1,  SERPINE1,  PLANH1
Swissprot
Calculated MW 45 kDa
Observed MW 47 kDa
The actual band is not consistent with the expectation.

Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include:

1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein.

2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes.

3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1.

4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids).

5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers.

If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane.

Concentration 1 mg/mL
Buffer Phosphate buffered solution, pH 7.2, containing 0.05% proclin 300.
Purification Method Antigen Affinity Purification
Conjugation Unconjugated
Storage Store at 4°C valid for 12 months or -20°C valid for long term storage, avoid freeze / thaw cycles.
Shipping The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.
background Serine protease inhibitor. Inhibits TMPRSS7. Is a primary inhibitor of tissue-type plasminogen activator (PlAT) and urokinase-type plasminogen activator (PlAU). As PlAT inhibitor,it is required for fibrinolysis down-regulation and is responsible for the controlled degradation of blood clots. As PlAU inhibitor,it is involved in the regulation of cell adhesion and spreading.Acts as a regulator of cell migration,independently of its role as protease inhibitor. It is required for stimulation of keratinocyte migration during cutaneous injury repair. It is involved in cellular and replicative senescence. Plays a role in alveolar type 2 cells senescence in the lung. Is involved in the regulation of cementogenic differentiation of periodontal ligament stem cells,and regulates odontoblast differentiation and dentin formation during odontogenesis.
Cat.No. Product Name Sizes
PDMR100067 Recombinant Rat Serpin E1/PAI-1 Protein(His Tag) 500μg , 100μg , 20μg , 1mg
Other Clones

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Unconjugated

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