Singlet Oxygen(1O2) Fluorometric Assay Kit (E-BC-F062)
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For research use only.
| Detection Principle |
Singlet oxygen (1O2) is a type of reactive oxygen species (ROS) generated through cellular metabolism, redox reactions, photosensitization, and other pathways. It can damage biological cellular components such as lipids, proteins, and nucleic acids, making it a subject of widespread attention across multiple fields. Although it is a potent oxidizing agent, intracellular antioxidant enzymes and antioxidants can inactivate it through corresponding mechanisms. Therefore, singlet oxygen and other ROS naturally generated in normal cells do not cause significant damage to organelles. This assay kit detects intracellular1O2 via fluorescence. The probe crosses the cell membrane into the cell, where it binds to 1O2 and emits fluorescence. Detection is performed using instruments such as flow cytometers and fluorescence microscopes. |
| Sample Type | Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microscope, Flow cytometry(FITC Channel) |
| Other Reagents Required | Methanol |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
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