Sirtuin 1 (SIRT-1) Activity Assay Kit (E-BC-F056)
For research use only.
| Detection Principle |
The Sirtuins (SIRT) family of proteins includes SIRT-1 and SIRT-7, which are histone deacetylases with nicotinamide adenine dinucleotide (NAD+) dependence and are commonly expressed in organs and tissues. Based on the amino acid sequence, the Sirtuins family proteins can be divided into four types: SIRT-1 and SIRT-3 are type 1, SIRT-4 is type 2, SIRT-5 is type 3, and SIRT-6 and SIRT-7 are type 4. SIRT-1 is widely distributed in organs and tissues, mainly present in the cytoplasm, and needs to enter the nucleus to function. Some drugs can regulate the expression of certain genes by inhibiting SIRT-1 entry into the nucleus. This kit uses the fluorescence resonance energy transfer (FRET) method for detection. After deacetylation by SIRT-1, the fluorescent substrate generates an activated fluorescent substrate, which can be decomposed by proteases to produce fluorescent substances. The maximum excitation wavelength of fluorescent substances is 340 nm, and the maximum emission wavelength is 440 nm. |
| Sample Type | Animal tissue,Cell |
| Detection Method | Fluorometric method |
| Detection Instrument | Fluorescence microplate reader (Ex/Em=340 nm/440 nm) |
| Research Area | Amino Acids And Proteins |
| Other Reagents Required | Normal saline (0.9% NaCl), Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.03 U/L |
| Detection Range | 0.03-9.66 U/L |
| Precision | inter-assay CV: 8.1-9.9% | intra-assay CV: 3.0-5.0% |
| Assay Time | 1 h 10 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Mouse brain tissue homogenate | 1 |
| 1 × 10^6 CHO cell | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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