Sorbitol Colorimetric Assay Kit (E-BC-K1156-M)
For research use only.
| Detection Principle |
Sorbitol, a six-carbon polyol, is involved in the sorbitol metabolic pathway (also known as the polyol pathway), one of the glucose metabolic pathways in cells. Abnormal activation of the sorbitol metabolic pathway is closely associated with chronic diabetic complications. Sorbitol dehydrogenase catalyzes the reaction of sorbitol with NAD+ to generate NADH. NADH reacts with WST-8 to produce the chromogenic formazan, which exhibits a characteristic absorption peak at 450 nm. The sorbitol content is calculated by measuring the absorbance at 450 nm. |
| Sample Type | Animal tissue,Plant tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm) |
| Research Area | Energy Metabolism , Metabolic Enzyme Activity , Carbohydrate Metabolism |
| Other Reagents Required | Double distilled water,Normal saline (0.9% NaCl) |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.001 mmol/L |
| Detection Range | 0.001-0.5 mmol/L |
| Precision | inter-assay CV: 4.0%-7.3% | intra-assay CV: 2.5%-3.6% |
| Sample Volume | 20 μL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 1 |
| 10% Mouse kidney tissue homogenization | 1 |
| 10% Mouse spleen tissue homogenization | 1 |
| 10% Mouse lung tissue homogenization | 1 |
| 10% Mouse brain tissue homogenization | 1 |
| 10% Mouse heart tissue homogenization | 1 |
| 10% Pothos leaf tissue | 1 |
| 1×10^6 HL-60 cells | 1 |
| 1×10^6 RAW264.7 cells | 1 |
| 1×10^6 A549 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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