SPCS2 Polyclonal Antibody (E-AB-18383)
For research use only.
| Verified Samples |
Verified Samples in WB: K562, Human placenta, Mouse adrenal gland, HT29, NIH/3T3, Rat lung Verified Samples in IHC: Human gastric cancer, Human breast cancer |
| Dilution | WB 1:500-1:2000, IHC 1:30-1:150 |
| Isotype | IgG |
| Host | Rabbit |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Immunogen | Fusion protein of human SPCS2 |
| Abbre | SPCS2 |
| Synonyms | Microsomal signal peptidase 25 kDa subunit, SPCS2, SPase 25 kDa subunit, Signal peptidase complex subunit 2, Signal peptidase complex subunit 2 homolog (S. cerevisiae) |
| Swissprot | |
| Calculated MW | 25 kDa |
| Observed MW |
Refer to figures
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Microsome membrane. Endoplasmic reticulum membrane. |
| Concentration | 1.1 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. |
| Purification Method | Antigen affinity purification |
| Research Areas | Signal transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | SPCS2 (signal peptidase complex subunit 2), also known as SPC25 or microsomal signal peptidase 25 kDa subunit, is a 226 amino acid multi-pass membrane protein that localizes to both the microsome and the endoplasmic reticulum (ER), and belongs to the SPCS (signal peptidase complex subunit) family. Existing as a component of the microsomal signal peptidase complex which consists of five members, SPCS2 removes signal peptides from nascent proteins as they are translocated into the lumen of the ER. The gene encoding SPCS2 is located on human chromosome 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. |
Other Clones
{{antibodyDetailsPage.numTotal}} Results
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
Other Formats
{{formatDetailsPage.numTotal}} Results
Unconjugated
-
{{item.title}}
Citations ({{item.publications_count}}) Manual MSDS
Cat.No.：{{item.cat}}
{{index}} {{goods_show_value}}
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.organization}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
