For research use only.
| Verified Samples |
Verified Samples in WB: U87-MG, HeLa, Jurkat, L-O2, 293T |
| Dilution | WB 1:1000-1:3000 |
| Clonality | Polyclonal |
| Immunogen | Recombinant fusion protein of human SPTBN1 (NP_003119.2). |
| Synonyms | ELF, HEL102, SPTB2, SPTBN1, betaSpII, spectrin beta |
| Swissprot | |
| Calculated MW | 251 kDa/253 kDa/274 kDa |
| Observed MW |
275 kDa
The actual band is not consistent with the expectation.
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Cellular Localization | Cell membrane and Cytoplasm, cytoskeleton, Cytoplasm, myofibril, sarcomere, M line, Colocalizes with ANK2 in a distinct intracellular compartment of neonatal cardiomyocytes. |
| Concentration | 1 mg/mL |
| Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Purification Method | Affinity purification |
| Research Areas | Tags, Cell Markers, Signal Transduction |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene. |
Other Clones
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