Starch Colorimetric Assay Kit (E-BC-K1209-M)
For research use only.
| Detection Principle |
Tyrosinase is widely distributed in higher animals and plants, primarily responsible for the browning of vegetables, beverages, and fruits, as well as the production of melanin in animals. Tyrosinase catalyzes the conversion of dopamine to dopaquinone. Dopaquinone forms a pink complex with MBTH. The tyrosinase activity in the sample can be calculated by measuring the rate of increase in OD value at 505 nm. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-510 nm) |
| Research Area | Plant Physiology |
| Other Reagents Required | Distilled or deionized water, 80% ethanol, 4 mol/L KOH、4 mol/L HCl, |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.01 mg/mL |
| Detection Range | 0.01-1.00 mg/mL |
| Precision | inter-assay CV: 3.0%-7.5% | intra-assay CV: 0.9%-2.4% |
| Sample Volume | 200-1000 μL |
| Assay Time | 50 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 1% dry corn seed tissue homogenization | 1-3 |
| 1% dry potato tissue homogenization | 2-5 |
| 1% dry wheat seed tissue homogenization | 2-5 |
| 1% dry rice tissue homogenization | 2-5 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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