STAT3 Monoclonal Antibody (E-AB-70314)
For research use only.
| Verified Samples |
Verified Samples in WB: HepG2, NIH/3T3, Mouse lung, Mouse brain, Rat brain |
| Dilution | WB 1:1000-1:3000 |
| Isotype | IgG |
| Host | Mouse |
| Reactivity | Human, Mouse, Rat |
| Applications | WB |
| Clonality | Monoclonal |
| Immunogen | Recombinant protein corresponding to Mouse Stat3 |
| Abbre | STAT3 |
| Synonyms | 1110034C02Rik, ADMIO, APRF, AW109958, Acute Phase Response Factor, Acute-phase response factor, DNA binding protein APRF, FLJ20882, HIES, MGC16063, Signal transducer and activator of t, Signal transducer and activator of transcription 3 (acute phase response factor) |
| Swissprot | |
| Calculated MW | 90 kDa |
| Observed MW |
90 kDa
Western blotting is a method for detecting a certain protein in a complex sample based on the specific binding of antigen and antibody. Different proteins can be divided into bands based on different mobility rates. The mobility is affected by many factors, which may cause the observed band size to be inconsistent with the expected size. The common factors include: 1. Post-translational modifications: For example, modifications such as glycosylation, phosphorylation, methylation, and acetylation will increase the molecular weight of the protein. 2. Splicing variants: Different expression patterns of various mRNA splicing bodies may produce proteins of different sizes. 3. Post-translational cleavage: Many proteins are first synthesized into precursor proteins and then cleaved to form active forms, such as COL1A1. 4. Relative charge: the composition of amino acids (the proportion of charged amino acids and uncharged amino acids). 5. Formation of multimers: For example, in protein dimer, strong interactions between proteins can cause the bands to be larger. However, the use of reducing conditions can usually avoid the formation of multimers. If a protein in a sample has different modified forms at the same time, multiple bands may be detected on the membrane. |
| Concentration | 2 mg/mL |
| Buffer | Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer, 1% protein protectant and 50% glycerol. |
| Purification Method | Affinity purification |
| Research Areas | Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Developmental Biology, Signal Transduction, Stem Cells |
| Clone No. | 4B2C9-3 |
| Conjugation | Unconjugated |
| Storage | Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. |
| Shipping | The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. |
| background | Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. |
Other Clones
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Unconjugated
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