Tannins Colorimetric Assay Kit (E-BC-K820-M)
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For research use only.
| Detection Principle |
Tannins are a class of natural polyphenolic compounds that are widely present in plants and have the property of binding with proteins, alkaloids and polysaccharides. With biological activities such as antioxidant, anti-inflammatory, and antibacterial effects, tannins have extensive applications in nutritional research, agricultural and plant sciences, and industrial fields. This kit determines the tannin content in the sample by measuring the OD value at 760 nm of the blue compound produced by the reaction between the sample and the chromogenic reagent under alkaline conditions. |
| Sample Type | Juice,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (740-780 nm, optimum wavelength: 760 nm) |
| Research Area | Plant Physiology Series |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.41 mg/L |
| Detection Range | 0.41-250 mg/L |
| Precision | inter-assay CV: 0.7%-4.4% | intra-assay CV: 0.4%-1.5% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Peach juice | 2-6 |
| Orange juice | 3-8 |
| Red wine | 5-10 |
| 10% Blueberry tissue homogenate | 2-5 |
| 10% Orange tissue homogenate | 1 |
| 10% Orange peel tissue homogenate | 2-4 |
| 10% Epipremnum aureum leaf tissue homogenate | 1 |
| 10% Scandent schefflera leaf tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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