Tissue Free Cholesterol (FC) Colorimetric Assay Kit (E-BC-K1104-M)
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For research use only.
| Detection Principle | Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content. |
| Sample Type | Animal tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-520 nm, optimum wavelength: 510 nm) |
| Research Area | Metabolic Diseases,Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.07 mmol/L |
| Detection Range | 0.07-24 mmol/L |
| Precision | inter-assay CV: 3.5%-5.2 % | intra-assay CV: 1.5%-1.9 % |
| Sample Volume | 5 μL(tissue homogenate) |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse heart tissue homogenate | 1 |
| 10% Mouse spleen tissue homogenate | 1 |
| 10% Mouse kidney tissue homogenate | 1 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Mouse lung tissue homogenate | 1 |
| 10% Porcine liver tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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