Total Glutathione (T-GSH)/Oxidized Glutathione (GSSG) Colorimetric Assay Kit (E-BC-K097-M)
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For research use only.
| Detection Principle | GSSG is reduced to GSH by glutathione reductase, and GSH can react with DTNB to produce GSSG and yellow TNB. The amount of total glutathione (GSSG+GSH) determines the amount of yellow TNB. Thus the total glutathione can be calculated by measuring the OD value at 412 nm. The content of GSSG can be determined by first removing GSH from the sample with GSH Scavenger and then applying the aforementioned reaction principle. |
| Synonyms | GSSG;T-GSH |
| Sample Type | Serum,Plasma,Animal tissue,Red blood Cell,cultured Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405-415 nm) |
| Research Area | Oxidative Stress , Cuproptosis , Ferroptosis |
| Other Reagents Required | PBS (0.01 mol/L, pH 7.4), Absolute ethanol |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.36 μmol/L |
| Detection Range | 0.36-30 μmol/L |
| Precision | inter-assay CV: 3.9% | intra-assay CV: 0.6% |
| Sample Volume | 100 μL |
| Assay Time | 1 h 40 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human plasma | 1 |
| 10% Rat liver tissue homogenate | 10-20 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat heart tissue homogenate | 10-20 |
| 10% Mouse brain tissue homogenate | 2-5 |
| HepG2 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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