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Dual-Reagent
Total Oxidant Status (TOS) Colorimetric Assay Kit (E-BC-K802-M)
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For research use only.
| Detection Principle | Under acid conditions, the oxidizing material in the sample can oxidizeFe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. In acidic media, the reaction product shows an absorbance peak near 590 nm. Under fixed experimental conditions (e.g., defined concentration range and reaction time), the absorbance correlates linearly with the oxidant concentration, enabling indirect quantification of the sample's total oxidizing capacity. |
| Synonyms | TOS |
| Sample Type | Cell,Tissue,Serum,Other liquid sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (580-590 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.5 μmol H<sub>2</sub>O<sub>2</sub> Equiv./L |
| Detection Range | 2.5-100 μmol H<sub>2</sub>O<sub>2</sub> Equiv./L |
| Precision | inter-assay CV: 3.5% | intra-assay CV: 2.3% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Porcine serum | 1 |
| Horse serum | 1 |
| Cynomolgus macaques serum | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 0.612 × 10^6 Molt-4 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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