Total Oxidant Status (TOS) Colorimetric Assay Kit (E-BC-K802-M)
For research use only.
| Detection Principle | Under acid conditions, the oxidizing material in the sample can oxidize Fe2+ to Fe3+, which binds highly with xylenol orange to produce a blue-purple complex. When the pH of solution is in the range of 2-3, its maximum absorption wavelength is around 590 nm, and the color depth is proportional to the content of oxidation substances in a certain concentration and a certain time, so as to indirectly calculate the total oxidation state of the sample. |
| Synonyms | TOS |
| Sample Type | Cell,Tissue,Serum,Other liquid sample |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (580-590 nm, optimum wavelength: 590 nm) |
| Research Area | Oxidative Stress |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.5 μmol H<sub>2</sub>O<sub>2</sub> Equiv./L |
| Detection Range | 2.5-100 μmol H<sub>2</sub>O<sub>2</sub> Equiv./L |
| Precision | inter-assay CV: 3.5% | intra-assay CV: 2.3% |
| Sample Volume | 20 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Porcine serum | 1 |
| Horse serum | 1 |
| Cynomolgus macaques serum | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 1 |
| 10% Rat spleen tissue homogenate | 1 |
| 0.612 × 10^6 Molt-4 cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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