Total Phenols Colorimetric Assay Kit (Plant samples) (E-BC-K354-M)
For research use only.
| Detection Principle | Under alkaline conditions, tungsten-molybdenum acid can be reduced by phenols and produce blue compounds, which has a characteristic absorption peak at 760 nm. The content of total phenols in sample can be calculated indirectly by measuring the absorbance at 760 nm. |
| Sample Type | Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (750-770 nm) |
| Research Area | Plant Physiology Series |
| Other Reagents Required | 60% Absolute ethanol |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 1.05 μg/mL |
| Detection Range | 1.05-148 μg/mL |
| Precision | inter-assay CV: 4.4% | intra-assay CV: 4.1% |
| Sample Volume | 20 μL(tissue homogenate) |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Epipremnum aureum tissue homogenate | 20-30 |
| Daucus carota tissue homogenate | 5-15 |
| Spinacia oleracea tissue homogenate | 15-25 |
| Leek tissue homogenate | 10-20 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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