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Total Sulfhydryl Group/Total Thiol (-SH) Colorimetric Assay Kit (E-BC-K265-M)

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96T $ 180.00
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For research use only.

Detection Principle Sulfhydryl compounds react with 5,5' -dithiobis (2-nitrobenzoic acid) under neutral or alkaline conditions to produce a yellow product which have a maximum absorption peak at 412 nm. Measure the OD value and calculate the total mercapto content indirectly.
Synonyms T-SH
Sample Type Serum,Plasma,Animal tissue
Detection Method Colorimetric method
Detection Instrument Microplate reader (410-420 nm, optimum wavelength: 412 nm)
Research Area Oxidative Stress
Other Reagents Required Absolute ethyl alcohol,Normal saline (0.9% NaCl)
Storage This product can be stored at 2-8°C for 12 months with shading light.
Valid Period 12 months
Sensitivity 9.91 μmol/L
Detection Range 9.91-1000 μmol/L
Precision inter-assay CV: 2.9% | intra-assay CV: 2.5%
Sample Volume 80 μL
Assay Time 20 min

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type Dilution Factor
Human serum 4-6
Human plasma 4-6
Human urine 1
Rabbit serum 3-5
Porcine serum 4-6
10% Rat liver tissue homogenate 4-6
10% Rat kidney tissue homogenate 4-6
10% Rat spleen tissue homogenate 4-6

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.

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