Transketolase(TKT) Activity Colorimetric Assay Kit (E-BC-K1151-M)
For research use only.
| Detection Principle |
Transketolase (TKT) is an important enzyme that catalyzes reactions in the pentose phosphate pathway, participating in the metabolism of various energy substances in the body (such as glucose, ribose, nucleotides, and lipids). TKT can improve blood glucose, glucose tolerance, and β-cell function by inhibiting the formation of advanced glycation end products, thereby reducing oxidative stress, inflammatory responses, atherosclerosis, endothelial dysfunction, and Tau protein phosphorylation, ultimately achieving the prevention and treatment of diabetes and its complications. TKT catalyzes the reaction of substrates such as xylulose and ribose to generate NADH. NADH reacts with a water-soluble tetrazolium salt to produce the chromogenic formazan, which exhibits a characteristic absorption peak at 450 nm. The activity of TKT is calculated by measuring the rate of increase in absorbance at 450 nm of the sample. |
| Sample Type | Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (445-455 nm) |
| Research Area | Energy Metabolism , Metabolic Enzyme Activity , Carbohydrate Metabolism |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.29 U/L |
| Detection Range | 0.29-28.57 U/L |
| Precision | inter-assay CV: 5.0%-7.2% | intra-assay CV: 3.5%-4.8% |
| Sample Volume | 20 μL |
| Assay Time | 1 h 20 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Rat liver tissue homogenization | 1-2 |
| 10% Mouse liver tissue homogenization | 1-2 |
| 10% Pig liver tissue homogenization | 1-2 |
| 10% Mouse Kidney tissue homogenization | 1 |
| 10% Mouse brain tissue homogenization | 1 |
| 10% Spinach leaf tissue | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
-
IF：{{item.impact}}
Journal：{{item.journal}} ({{item.year}})
DOI：{{item.doi}}Reactivity：{{item.species}}
Sample Type：{{item.sample_type}}
-
Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}}
No files available. Please enter the correct identification code in the search area to query the relevant documents.
| Title | Status | Valid until | Action | |
|---|---|---|---|---|
| {{v.文件名}} | {{v.status_str}} | {{v.timestamp_str}} | Download | / |
*Note：Please download the relevant files within the validity period. After expiration, file preview and download will not be available on the page.
Contact with Us
Houston, Texas, 77079, USA
