Trypsin Activity Assay Kit (E-BC-K843-M)

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All	Size	Price	Qty
	96T	$ 360.00
	48T	$ 240.00

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Detection Principle	Trypsin catalyzes the substrate to form p-nitroaniline, which has a certain light absorption value at 400-410 nm wavelength. Since the absorbance of p-NA is proportional to the content, the activity of trypsin can be calculated by measuring the p-NA produced per unit time.
Sample Type	Animal tissue
Detection Method	Colorimetric method
Detection Instrument	Microplate reader (400-410 nm, optimum wavelength: 405 nm)
Research Area	Amino Acids And Proteins
Storage	This product can be stored at -20°C for 12 months with shading light.
Valid Period	12 months
Sensitivity	1.86 U/L
Detection Range	1.86-100.65 U/L
Precision	inter-assay CV: 7.2-11.2% \| intra-assay CV: 3.9-5.1%
Sample Volume	20 μL(cell homogenate)

Cat.No.	Product Name	Sizes
E-BC-K844-M	Pepsin Activity Assay Kit	96T

The recommended dilution factor for different samples is as follows (for reference only):

Sample Type	Dilution Factor
10% Mouse small intestine tissue homogenate	2-5
10% Mouse large intestine tissue homogenate	2-5
10% Rat intestine tissue homogenate	2-5
10% Rat chyme tissue homogenate	2-5

The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.