Uncoated Human CCL15/MIP-5(C-C motif chemokine 15/Macrophage inflammatory protein 5) ELISA Kit (E-UNEL-H0397)
Product Summary
The new series of Elabscience® Uncoated ELISA kits contain the basic components required for the ELISA experiments. For customers who have rich experience and can establish their own ELISA system, uncoated kit is an ideal choice to save costs.
| Detection Range | 31.25-2000 pg/mL |
| Sample Volume | 100 μL |
| Total Assay Time | 24 h |
| Reactivity | Human |
| Specificity | This kit recognizes Human CCL15/MIP-5 in samples.No significant cross-reactivity or interference between Human CCL15/MIP-5 and analogues was observed |
| Recovery | 80%-120% |
| Sample Type | Serum, plasma and other biological fluids |
| Detection Method | Colorimetric method, ELISA, Sandwich |
| Assay Type | Sandwich-ELISA |
| Size | 96T*5 / 96T*15 |
| Storage | -20℃ |
| Expiration Date | 12 months |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CCL15/MIP-5. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CCL15/MIP-5 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CCL15/MIP-5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CCL15/MIP-5. You can calculate the concentration of Human CCL15/MIP-5 in the samples by comparing the OD of the samples to the standard curve.
Background
| Uniport ID | Q16663 |
| Research Area | Cardiovascular , Immunology |
| Cat.No. | Product Name | Clone No. |
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