Urease Activity Colorimetric Assay Kit (E-BC-K1405-M)
For research use only.
| Detection Principle |
Urease is an oligomeric enzyme containing divalent nickel ions with absolute specificity. It specifically catalyzes the hydrolysis of urea to release ammonia and carbon dioxide, and plays an important role in the nitrogen cycle in nature. Urease is mainly extracted from the seeds of leguminous plants such as sword bean and soybean, and microorganisms such as Arthrobacter can also secrete urease with similar functions. Detection principle of this kit: under the catalysis of urease, urea is hydrolyzed to generate carbon dioxide (CO2) and ammonia (NH3). The generated ammonia reacts with hypochlorite (OCl-) to form monochloramine, which further reacts with phenol to form blue indophenols. Indophenols has a maximum absorption peak at around 650 nm, and within a certain range, the amount of indophenols generated is proportional to the urease activity in the sample. |
| Sample Type | Plant tissue,Microorganism |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (640-660 nm) |
| Research Area | Plant Physiology , Metabolic Enzyme Activity |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.13 U/L |
| Detection Range | 0.13-66.67 U/L |
| Precision | inter-assay CV: 1.0%-2.0% | intra-assay CV: 0.7%-1.4% |
| Sample Volume | 10 μL |
| Assay Time | 1 h 25 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Sword bean seed | 1000-5000 |
| Edamame seed | 1000-5000 |
| Mung bean | 1 |
| Red kidney bean | 1 |
| Pumpkin seed | 1 |
| Pine nut | 1 |
| Peanut kernel | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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