Uric Acid (UA) Colorimetric Assay Kit (Enzyme Method) (E-BC-K897-M)
For research use only.
| Detection Principle | Uric Acid produces allantoin, CO2 and H2O2 under the action of Uricase, and H2O2 can produce red quinone imide compounds under the action of color developer, whose color depth is proportional to the H2O2 content, and has the maximum absorption at 510 nm wavelength, so as to calculate the uric acid content. |
| Sample Type | Serum,Plasma |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (500-520 nm, optimum wavelength: 510 nm) |
| Research Area | Liver And Renal Function |
| Storage | This product can be stored at 2-8°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 2.95 mg/L |
| Detection Range | 2.95-100.00 mg/L |
| Precision | inter-assay CV: 2.7-5.1% | intra-assay CV: 1.3-1.8% |
| Sample Volume | 5 μL |
| Assay Time | 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Human plasma | 1 |
| Rabbit serum | 1 |
| Rat plasma | 1 |
| Bovine serum | 1 |
| Mouse plasma | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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