Uridine Diphosphate Glucose Pyrophosphorylase (UGP) Activity Assay Kit (E-BC-K857-M)
For research use only.
| Detection Principle |
Uridine diphosphate glucose pyrophosphorylase (UGP) catalyzes glucose activation before glycogen synthesis, synthesizing 1-phosphate glucose and UTP molecules into UDP-glucose (UDPG). UDPG is the main form of activating enzyme in higher plants and animals, and participates in the anabolic metabolism of glycogen, sucrose and cellulose as a glucosyl donor. Uridine diphosphate glucose pyrophosphorylase (UGP) catalyzes the synthesis of 1-phosphoglucose in the sample into UDP-glucose, and UDP-glucose is catalyzed by the enzyme to produce a color producing substance, which has a maximum absorption peak at about 450 nm. The enzyme activity can be calculated by the increase of OD value at 450 nm. |
| Sample Type | Animal tissue,Plant tissue |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (440-460 nm, optimum wavelength: 450 nm) |
| Research Area | Glycolysis And Lipid Metabolism |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.5 U/L |
| Detection Range | 0.5-70.0 U/L |
| Precision | inter-assay CV: 2.3-3.9% | intra-assay CV: 1.0-3.2% |
| Sample Volume | 40 μL(tissue homogenate) |
| Assay Time | 35 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Corn tissue homogenate | 180-200 |
| 10% Patato tissue homogenate | 30-40 |
| 10% Sweet patato tissue homogenate | 100-120 |
| 10% Corn husk tissue homogenate | 35-45 |
| 10% Epipremnum aureum tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat liver tissue homogenate | 100-120 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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